rabbit anti psyk y352 65e4 id catalog (Cell Signaling Technology Inc)
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rabbit anti psyk y352 65e4 id catalog
Rabbit Anti Psyk Y352 65e4 Id Catalog, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+psyk+y352+65e4+id+catalog/Phospho-Zap-70+(Tyr319)%2FSyk+(Tyr352)+Rabbit+mAb/bio_rxiv__2025__04__16__648985-179-7-4
Average 95 stars, based on 126 article reviews
Rabbit Anti Psyk Y352 65e4 Id Catalog, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+psyk+y352+65e4+id+catalog/Phospho-Zap-70+(Tyr319)%2FSyk+(Tyr352)+Rabbit+mAb/bio_rxiv__2025__04__16__648985-179-7-4
Average 95 stars, based on 126 article reviews
rabbit anti psyk y352 65e4 id catalog - by Bioz Stars,
2026-09
95/100 stars
Images
1) Product Images from "Steady-state phosphorylation of SHIP1 by Lyn restricts macrophage activation in the absence of a phagocytic synapse"
Article Title: Steady-state phosphorylation of SHIP1 by Lyn restricts macrophage activation in the absence of a phagocytic synapse
Journal: bioRxiv
doi: 10.1101/2025.04.16.648985
Figure Legend Snippet: (A-C) Representative confocal micrographs showing a single z slice (∼280 nm) of IFN-γ-primed Csk AS BMDMs stained for total Lyn (yellow) and pSyk Y352 (cyan) after 3 min treatments with (A) medium only (untreated), (B) 3-IB-PP1, or (C) depleted zy-mosan (zym dep ). Fluorescence in the DAPI channel shows an internalized zym dep (magenta). Scale bars: 5 μm (D) Interaction area for pSyk Y352 clusters formed spontaneously during 3-IB-PP1 treatment and semi-contiguous interaction area per phagocytosed zym dep particle. Points are single-punctum values combined from 5 cells, shown with standard error of the mean (SEM). Significance (Sig.) in all panels assessed via nonparametric t test and Kolmogorov-Smirnov test: *** P = 0.0001. Inset: view of the lower range of 3-IB-PP1-induced pSyk clustering, showing the preponderance of nanoclusters within the distribution.
Techniques Used: Staining, Fluorescence
Figure Legend Snippet: Densitometry quantification of protein species in immunoblotted lysates from IFN-γ-primed Csk AS BMDMs after 5 min treatment with 3-IB-PP1 or zym dep probed by immunoblot for (A) pSyk Y352 , (B) pPLCγ2 Y1217 , (C) pPI3K p85/p55 (D) immunoprecipitated Ras-GTP, (E) pErk1/2 Y202/T204 , and (F) pAkt S473 . Error: SEM; n=9-14 for 3-IB-PP1 and 11-15 for zym dep . Sig. assessed via non-parametric t test and Kolmogorov-Smirnov test: * P = 0.0216, *** P = 0.0007, ** P < 0.0001.
Techniques Used: Western Blot, Immunoprecipitation
Figure Legend Snippet: (A-D) Immunoblots and quantifications of lysates from IFN-γ-primed Lyn +/+ and Lyn KO BMDMs probed for phosphorylated (A) Syk Y352 , (B) Erk1/2 T202/Y204 , (C) Akt S473 , and (D) SHIP1 Y1020 . β-Actin is a visual loading control, and quantifications were normalized to total protein content in the gel lane. Data are shown relative to the signal in Lyn +/+ . n=10-15 for Lyn +/+ and 6-7 for Lyn KO . Error bars: SEM. Sig. nonparametric, unpaired t with Kolmogorov-Smirnov test, *P=0.0152, **P≥0.007, ***P=0.0006 and ****P≤0.0001. (E) Representative images of PIP 3 (yellow) and nuclear staining (cyan) in IFN-γ -primed BMDMs. (F) Quantification of the geometric mean fluorescence intensity (gMFI) of PIP 3 staining in Lyn KO relative to Lyn +/+ BMDMs. Data points reflect the mean and SEM of 3-4 independent wells from 4 biological replicates. Sig. nonparametric, unpaired t test with Kolmogorov-Smirnov test, *** P<0.0006.
Techniques Used: Western Blot, Control, Staining, Fluorescence
Figure Legend Snippet: Immunoblots and quantifications of (A-B) pSyk Y352 , (C-D) pErk1/2 T202/Y204 , (E-F) pAkt S473 , and (G-H) pSHIP1 Y1020 in IFN-γ-primed Csk AS (Lyn +/+ ) or Csk AS Lyn KO (Lyn KO ) BMDMs treated with (A, C, E, G) 3-IB-PP1 or (B, D, F, H) zym dep , corrected for total protein in each gel lane and shown relative to t=0 of Lyn +/+ ; n=10-11 for Lyn +/+ , 4-7 for Lyn KO . Error bars: SEM. Sig. via two-way ANOVA with Sidak’s multiple comparisons test: * P = 0.0143, ** P = 0.0041, **** P<0.0001.
Techniques Used: Western Blot
Figure Legend Snippet: Immunoblots and quantification of (A) pSHIP1 Y1020 , (B) pAkt S473 , (C) pSyk Y352 , and (D) pErk T202/Y204 in CskAS (Hck +/+ Fgr +/+ ) and Csk AS Hck KO Fgr KO (Hck KO Fgr KO ) BMDMs, corrected for the total protein in each lane and shown relative to t=0 of Hck +/+ Fgr +/+ . Error bars: SEM, n=10-11 for Hck +/+ Fgr +/+ and n=4 for Hck KO Fgr KO . Sig. 2-way ANOVA with Sidak’s multiple comparisons test: * P = 0.0499.
Techniques Used: Western Blot
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Staining:Article Title: Steady-state phosphorylation of SHIP1 by Lyn restricts macrophage activation in the absence of a phagocytic synapse Article Snippet: Densitometry quantification was performed in ImageStudio (LI-COR), as described previously [ ].Densitometry quantification was performed in ImageStudio (LI-COR), as described previously [ ].. Antibodies were obtained from Fluorescence:Article Title: Steady-state phosphorylation of SHIP1 by Lyn restricts macrophage activation in the absence of a phagocytic synapse Article Snippet: Densitometry quantification was performed in ImageStudio (LI-COR), as described previously [ ].Densitometry quantification was performed in ImageStudio (LI-COR), as described previously [ ].. Antibodies were obtained from Western Blot:Article Title: Steady-state phosphorylation of SHIP1 by Lyn restricts macrophage activation in the absence of a phagocytic synapse Article Snippet: Densitometry quantification was performed in ImageStudio (LI-COR), as described previously [ ].Densitometry quantification was performed in ImageStudio (LI-COR), as described previously [ ].. Antibodies were obtained from Immunoprecipitation:Article Title: Steady-state phosphorylation of SHIP1 by Lyn restricts macrophage activation in the absence of a phagocytic synapse Article Snippet: Densitometry quantification was performed in ImageStudio (LI-COR), as described previously [ ].Densitometry quantification was performed in ImageStudio (LI-COR), as described previously [ ].. Antibodies were obtained from Control:Article Title: Steady-state phosphorylation of SHIP1 by Lyn restricts macrophage activation in the absence of a phagocytic synapse Article Snippet: Densitometry quantification was performed in ImageStudio (LI-COR), as described previously [ ].Densitometry quantification was performed in ImageStudio (LI-COR), as described previously [ ].. Antibodies were obtained from |